朱作言文集（全2冊）

定　價：￥880.00

作　者：	汪亞平等著
出版社：	科學出版社
叢編項：
標　簽：	暫缺

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ISBN：	9787030604613	出版時間：	2019-03-01	包裝：	精裝
開本：	16開	頁數：	1404	字數：

內容簡介

　　朱作言先生研究團隊于20 世紀80 年代初期農業(yè)動物基因工程研究，在1985 年發(fā)表了世界第一篇魚類基因轉移研究文章，享有世界聲譽?！吨熳餮晕募ㄈ?冊）》從該團隊發(fā)表的200 多篇文章中，選取了100 篇編輯成冊，分上卷和下卷出版?！吨熳餮晕募ㄈ?冊）》的主線是基于轉基因魚理論模型的建立、轉基因鯉研制及品系選育、轉植基因功能，以及轉基因魚食用和生態(tài)安全研究。內容共包括7 個部分：第一部分和第二部分建立了轉基因魚研究的完整理論模型，并以此為基礎成功研制轉“全魚”生長激素基因黃河鯉家系，系統(tǒng)揭示了轉植基因的整合、表達和遺傳規(guī)律。第三部分從攝食、代謝、生長、免疫和游泳行為等方面，詳細描述了轉基因黃河鯉與野生黃河鯉的生物學特性。第四部分系統(tǒng)評估了轉基因魚在實驗室及模擬自然環(huán)境條件下的生態(tài)學效應。第五部分系統(tǒng)研究了魚類生長激素對性腺發(fā)育的調控作用、育性控制技術及研制不育轉基因魚的策略。第六部分包括草魚全基因組草圖繪制、草魚病原病毒分子生物學和抗病草魚基因工程育種的基礎研究。第七部分主要以斑馬魚為模型，對魚類基因工程的生物學原理、技術拓展和應用進行了全方位的探討。為了便于廣大讀者閱讀，英文文章后面均附有中文摘要。

作者簡介

暫缺《朱作言文集（全2冊）》作者簡介

圖書目錄

目錄
上卷
第一部分魚類基因轉移模型建立
Novel Gene Transfer into the Fertilized Eggs of Goldfish (Carassius auratus L 1758) 3
人生長激素基因在泥鰍受精卵顯微注射轉移后的生物學效應 8
Introduction of Novel Genes into Fish 11
轉基因魚模型的建立 23
第二部分外源基因的整合與表達
The β-Actin Gene of Carp (Ctenopharyngodon idella) 35
Isolation and Characterization of β-Actin Gene of Carp (Cyprinus carpio) 37
Primary-Structural and Evolutionary Analyses of the Growth-Hormone Gene from Grass
Carp (Ctenopharyngodon idellus) 54
Time Course of Foreign Gene Integration and Expression in Transgenic Fish Embryos 64
Transgenes in F4 pMThGH-Transgenic Common Carp (Cyprinus carpio L ) are Highly
Polymorphic 73
Sequences of Transgene Insertion Sites in Transgenic F4 Common Carp 84
Characterization of Transgene Integration Pattern in F4 hGH-Transgenic Common Carp
(Cyprinus carpio L ) 87
Integration of Double-Fluorescence Expression Vectors into Zebrafish Genome for the
Selection of Site-Directed Knockout/Knockin 101
Site-Directed Gene Integration in Transgenic Zebrafish Mediated by Cre Recombinase
Using A Combination of Mutant Lox Sites 114
Inhibition of No Tail (ntl) Gene Expression in Zebrafish by External Guide Sequence
(EGS) Technique 128
Non-Homologous End Joining Plays A Key Role in Transgene Concatemer Formation in
Transgenic Zebrafish Embryos 137
Integration Mechanisms of Transgenes and Population Fitness of GH Transgenic Fish 157
Gene Transfer and Mutagenesis Mediated by Sleeping Beauty Transposon in Nile Tilapia
(Oreochromis niloticus) 172
Targeted Expression in Zebrafish Primordial Germ Cells by Cre/LoxP and Gal4/UAS
Systems 189
Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in
Zebrafish Using TALENs 211
Efficient Ligase 3-Dependent Microhomology-Mediated End Joining Repair of DNA
Double-Strand Breaks in Zebrafish Embryos 225
第三部分外源基因表達的生理功能
Hormonal Replacement Therapy in Fish: Human Growth Hormone Gene Function in
Hypophysectomized Carp 249
Food Consumption and Energy Budget in MThGH-Transgenic F2 Red Carp (Cyprinus
carpio L red var ) 263
Whole-Body Amino Acid Pattern of F4 Human Growth Hormone Gene-Transgenic Red
Common Carp (Cyprinus carpio) Fed Diets with Different Protein Levels 269
Transgene for Growth Hormone in Common Carp (Cyprinus carpio L ) Promotes Thymus
Development 276
Effects of the “All-Fish” Growth Hormone Transgene Expression on Non-Specific
Immune Functions of Common Carp， Cyprinus carpio L 287
Rapid Growth Cost in “All-Fish” Growth Hormone Gene Transgenic Carp: Reduced
Critical Swimming Speed 298
Growth and Energy Budget of F2 ‘All-Fish’ Growth Hormone Gene Transgenic Common
Carp 307
Fast-Growing Transgenic Common Carp Mounting Compensatory Growth 321
Metabolism Traits of ‘All-Fish’ Growth Hormone Transgenic Common Carp (Cyprinus
carpio L ) 334
Enzyme-Linked Immunosorbent Assay of Changes in Serum Levels of Growth
Hormone (cGH) in Common Carps (Cyprinus carpio) 350
The Hematological Response to Exhaustive Exercise in ‘All-Fish’ Growth
Hormone Transgenic Common Carp (Cyprinus carpio L ) 362
Acute and Chronic Un-Ionized Ammonia Toxicity to ‘All-Fish’ Growth Hormone
Transgenic Common Carp (Cyprinus carpio L ) 376
Effects of Growth Hormone (GH) Transgene and Nutrition on Growth and Bone
Development in Common Carp 386
Increased Food Intake in Growth Hormone-Transgenic Common Carp (Cyprinus carpio L )
May Be Mediated by Upregulating Agouti-Related Protein (AgRP) 401
第四部分轉基因黃河鯉的生態(tài)安全研究
Progress in the Evaluation of Transgenic Fish for Possible Ecological Risk and Its
Containment Strategies 423
Reduced Swimming Abilities in Fast-Growing Transgenic Common Carp Cyprinus carpio
Associated with Their Morphological Variations 433
Elevated Ability to Compete for Limited Food Resources by ‘All-Fish’ Growth
Hormone Transgenic Common Carp Cyprinus carpio 446
Increased Mortality of Growth-Enhanced Transgenic Common Carp (Cyprinus carpio L )
Under Short-Term Predation Risk 462
Behavioral Alterations in GH Transgenic Common Carp May Explain Enhanced
Competitive Feeding Ability 471
Risk-Taking Behaviour May Explain High Predation Mortality of GH-Transgenic
Common Carp Cyprinus carpio 489
Transgenic Common Carp Do Not Have the Ability to Expand Populations 506
Rapid Growth Increases Intrinsic Predation Risk in Genetically Modified Cyprinus carpio:
Implications for Environmental Risk 518
第五部分魚類生殖調控與轉基因魚的育性控制研究
A Perspective on Fish Gonad Manipulation for Biotechnical Applications 535
Cloning and Expression Analysis in Mature Individuals of Two Chicken Type-II GnRH
(cGnRH-II) Genes in Common Carp (Cyprinus carpio) 546
Identification and Characterization of A Novel Splice Variant of Gonadotropin α
Subunit in the Common Carp Cyprinus carpio 560
Antisense for Gonadotropin-Releasing Hormone Reduces Gonadotropin